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To achieve this, the inventors produced a big plasmid (˜twelve kb) exchanging the p15a-dependent origin of replication with the primase origin. This plasmid targets the lacZ gene (p1322, SEQ ID NO: 25) and likewise has a chloramphenicol marker. Since it was disregarded if the RBS power would wish to be modified to copy a sizable plasmid, the inven… Read More

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